

We concluded that Analixity is an open-source software as reliable and effective as a commercial software.īehavior Elevated plus maze Image processing Open source Software Video analysis.Ĭopyright © 2021 Elsevier B.V. We did not find statistically significant differences in the quantification of parameters such as time spent in open arms, time spent in closed arms, time spent in center zone, number of closed arms, open arms entries, and anxiety index. In addition, we compared the results obtained by Analixity with results obtained by the commercial software ANY-maze. We did not find statistically significant differences. For validation, we compared results obtained by Analixity with results obtained by manual analysis. Analixity generates an Excel file with the quantified behavioral variables, such as time spent in open and closed arms and in the center zone, number of entries to each zone and total distance traveled during the test. Analixity is an adaptable multiplatform open-source system. Here, we introduce Analixity, a video processing software for the elevated plus maze test (EPM), in which quantification of behavioral parameters is automatic, reducing the time spent in analysis and solving the variability problem. The assessment of the researcher during the quantification of such parameters can introduce variability among experimental conditions or among sessions of analysis. View your reports in the format you needtext, graphs, statistical analysis and/or. The video tracking software offers more than 3. ANY- maze’s many reporting options allow you to view and analyze your data in almost any way desired. And even if you do, you could implement a random chance on a open loop maze so that some of the time, it takes the other way.įrom that list, now, all you need to do is to climb through it and determine which path you took the last time at a branch, and take the other one.Manual analysis of behavioral tests in rodents involves inspection of video recordings by a researcher that assesses rodent movements to quantify parameters related with a behavior of interest. ANY- maze is a video tracking system designed to automate testing in a wide range of behavioral apparatus. Keep the last 50 moves in memory - this provides enough history to not keep taking the wrong way. You can retain this array to a limited size if you use a ring buffer.

But this provides a list you can traverse and repeat UP TO the side of memory you have available. You can use floats for each of these, assuming your program isn't too huge. (angle of travel, distance traveled, x position, y position) 90 deg, 0 m, 0, 0 - Initial positionġ80 deg, 1m, 1,2 - travel 1m to the left And you can measure how much you turn and how far you go.

You know that you start "north" (you set it as the definition). Use odometry and calculate what direction and how far.
